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Generalized linearization of kinetics of glucose isomerization to fructose by immobilized glucose isomerase

✍ Scribed by Emilio Palazzi; Attilio Converti


Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
161 KB
Volume
63
Category
Article
ISSN
0006-3592

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✦ Synopsis


The kinetic parameters of both glucose isomerization to fructose and immobilized glucose isomerase (GI) inactivation calculated under different conditions are compared and discussed. Utilizing these figures, the possibility of generalizing a linear model, previously proposed for the kinetics of glucose isomerization by immobilized glucose isomerase, is investigated, so as to apply them to whole ranges of temperature and concentrations of actual interest in industrial processes. The proposed model is a satisfactory approximation of the more involved Briggs-Haldane approach and substantially simplifies the problem of optimizing an industrial fixed-bed column for high-fructose corn syrup (HFCS) production.


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Five different types of reactors were employed for glucose isomerization using shrimp shell as the support on which to immobilize the glucose isomerase. The Michaelis-Menten constants and effective diffusivity of glucose in the immobilized enzyme bed were experimentally determined and used in a theo