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Purification, and phosphorylation in vivo and in vitro, of phosphoenolpyruvate carboxykinase from cucumber cotyledons

✍ Scribed by Robert P Walker; Richard C Leegood

Book ID
115930605
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
410 KB
Volume
362
Category
Article
ISSN
0014-5793

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πŸ“œ SIMILAR VOLUMES

Phosphoenolpyruvate carboxykinase from h
Phosphoenolpyruvate carboxykinase from higher plants: Purification from cucumber and evidence of rapid proteolytic cleavage in extracts from a range of plant tissues
✍ Robert P. Walker; Stephen J. Trevanion; Richard C. Leegood πŸ“‚ Article πŸ“… 1995 πŸ› Springer-Verlag 🌐 English βš– 640 KB

Phosphoenolpyruvate carboxykinase (PEPCK) was purified 600-fold to homogeneity from the cotyledons of cucumber (Cucumis sativus L.) and a polyclonal antiserum raised. After sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) the purified preparation contained a single polypeptide of