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Purification and partial characterization of α-l-arabinofuranosidase produced byThermomonospora fusca

✍ Scribed by M. Tuncer; A. S. Ball


Publisher
Springer Netherlands
Year
2003
Tongue
English
Weight
751 KB
Volume
48
Category
Article
ISSN
0015-5632

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✍ Jean-Marc Brillouet; Jean-Claude Moulin; Eduardo Agosin 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 877 KB

A white-rot fungus Dichomitus squalens, when grown on 1% wheat-straw glucuronoarabinoxylan under aerated submerged conditions, secreted an a-~arabinofuranosidase (4.3 nkat/mL). The enzyme was purified 70-fold by ammonium sulfate precipitation, chromatofocusing on PBE 94, gel filtration on Ultrogel A