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Purification and partial characterization of R5, R5X4, and X4 HIV-1 subtype C envelope glycoproteins expressed in insect cells

✍ Scribed by Sibusiso P. Nkosi; Henk Huismans; Maria A. Papathanasopoulos

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
156 KB
Volume
76
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

Biological properties of four recombinant HIV‐1 subtype C envelope glycoproteins from viruses with different phenotypic characteristics (CCR5 and/or CXCR4‐utilizing) were investigated. The gp160 genes were cloned, expressed in Spodoptera frugiperda insect cells, purified, and their biological characteristics were examined. The conformational and functional integrity of the HIV‐1 subtype C rgp160 was intact since they reacted with the A32, C11, IgG1b12, 7B2, and 17b conformational dependant monoclonal antibodies (MAb), sCD4, and patient sera. Baculovirus derived rgp160 can be used for further structural, functional, antigenic, and immunological studies. J. Med. Virol. 76:459–463, 2005. Β© 2005 Wiley‐Liss, Inc.

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