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Purification and partial characterization of citrate synthase from Phaseolus vulgaris mitochondria

✍ Scribed by Gerald A. Greenblatt; Igot V. Sarkissian

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
492 KB
Volume
12
Category
Article
ISSN
0031-9422

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An acetohydroxyacid synthase (EC 4.1.3.18) which synthesizes a-acetolactate from pyruvate has been isolated from two different CIaviceps purpurea strains. A purification of about 142-fold was achieved by ammonium sulfate fractionation and the use of Sepharose 6B and DEAE-Sepharose CL-6B columns. The