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Purification and characterization of cysteine synthases from Citrullus vulgaris

✍ Scribed by Fumio Ikegami; Masakazu Kaneko; Hiromi Kamiyama; Isamu Murakoshi

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
502 KB
Volume
27
Category
Article
ISSN
0031-9422

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Acetohydroxyacid synthase from Claviceps
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✍ Dr. Walter Maier; Rajesh Luthra; Detlef GrΓΆger πŸ“‚ Article πŸ“… 1989 πŸ› John Wiley and Sons 🌐 English βš– 466 KB

An acetohydroxyacid synthase (EC 4.1.3.18) which synthesizes a-acetolactate from pyruvate has been isolated from two different CIaviceps purpurea strains. A purification of about 142-fold was achieved by ammonium sulfate fractionation and the use of Sepharose 6B and DEAE-Sepharose CL-6B columns. The