Purification and properties of endo-(1→4
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Kunio Ohmiya; Keiko Maeda; Shoichi Shimizu
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Article
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1987
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Elsevier Science
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English
⚖ 875 KB
An enzyme active against 0-(carboxymethyl)cellulose (CMC) was purified from a synthetic medium containing ball-milled cellulose wherein Ruminococcus albus had been cultivated for 70 h. After 570-fold purification, a homogeneous enzyme was obtained in a yield of 3%. The enzyme degraded CMC (molecular