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Purification and kinetic characterization of hexokinase and glucose-6-phosphate dehydrogenase from Schizosaccharomyces pombe

✍ Scribed by Tsai, C Stan; Chen, Q

Book ID
127002857
Publisher
NRC Research Press
Year
1998
Tongue
English
Weight
149 KB
Volume
76
Category
Article
ISSN
0829-8211

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Glucose-6-phosphate dehydrogenase was purified to homogeneity from testes and kidneys of the inbred strain of mice (DBA/2J) by a simple two-step affinity column procedure. This involved the sequential application of 8-(6-aminohexyl)-amino-AMP- and -2', 5'-ADP-Sepharose columns and biospecific elutio