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Purification and Kinetic Characterization of Glucose-6-phosphate Dehydrogenase from Dictyostelium discoideum

✍ Scribed by Jacqueline M. Reimers; Qi Huang; Kathy R. Albe; Barbara E. Wright

Book ID
115600391
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
240 KB
Volume
17
Category
Article
ISSN
0147-5975

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Glucose-6-phosphate dehydrogenase was purified to homogeneity from testes and kidneys of the inbred strain of mice (DBA/2J) by a simple two-step affinity column procedure. This involved the sequential application of 8-(6-aminohexyl)-amino-AMP- and -2', 5'-ADP-Sepharose columns and biospecific elutio