The availability of bacterial ribosomal subunits has allowed the detailed study of the mechanisms and control of protein synthesis in prokaryotes (1,2). Techniques for the isolation of active subunits of eukaryote ribosomes are now available (3,4) ; the separation of subunits is achieved by gradien
Purification and isolation of active mammalian ribosomal subunits
โ Scribed by Sherman R. Dickman
- Publisher
- John Wiley and Sons
- Year
- 1969
- Tongue
- English
- Weight
- 177 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
We have partially purified active delta and epsilon subunits of the E. coli membranebound MgZf-ATPase (ECF1). Treating purified ECFl with 50% pyridine precipitates the major subunits (a, 0, and y ) of the enzyme, but the two minor subunits (6 and E ) , which are present in relatively small amounts,
## Eukaryotic genes are divided into three categories according to the machineries by which they are transcribed. Ribosomal RNA genes (rDNA) are the only ones that are transcribed by RNA polymerase Iand are under diflerent control from other genes transcribed by RNA polymerase 11 or III. None the le