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Purification and comparison of native and recombinant tRNA-guanine transglycosylases from Methanosarcina acetivorans

✍ Scribed by Nomura, Yuichiro; Onda, Yumiko; Ohno, Satoshi; Taniguchi, Hiroki; Ando, Kaori; Oka, Natsuhisa; Nishikawa, Kazuya; Yokogawa, Takashi


Book ID
121961136
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
652 KB
Volume
88
Category
Article
ISSN
1046-5928

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Purification, crystallization, and preli
✍ Romier, Christophe; Ficner, Ralf; Reuter, Klaus; Suck, Dietrich πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 399 KB πŸ‘ 2 views

The tRNA modifying enzyme tRNA-guanine transglycosylase (Tgt) catalyzes the exchange of guanine in the first position of the anticodon with the queuine precursor 7-aminomethyl-7-deazaguanine. Tgt from Zymomo-na8 mobilis has been purified by crystallization and further recrystallized to obtain single