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Purification and characterization of the FokI restriction endonuclease

โœ Scribed by Kaczorowski, Tadeusz; Skowron, Piotr; Podhajska, Anna J.


Book ID
122740257
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
921 KB
Volume
80
Category
Article
ISSN
0378-1119

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โœ Krishna Baski; George W. Rushizky ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 514 KB

The restriction endonuclease Pal1 was purified from Providencia alcalifaciens 1650-fold with a yield of 33%. The purified protein moved as a single band upon polyacrylamide gel electrophoresis. When this was carried out in the presence of sodium dodecyl sulfate, a molecular weight of 31,000 was obta