It is known that two gluconokinases are inducibly expressed during the utilization of gluconate by E. coli. One is therrnoresistant (activity stable for 3 h at 30 "C) and the other thermosensitive (losses 75% or more of its activity under the above conditions). The thermoresistant gluconokinase (EC
Purification and characterization of the Escherichia coli thermoresistant gluconokinase encoded by the gntK gene
β Scribed by Hanae Izu; Osao Adachi; Mamoru Yamada
- Book ID
- 115932850
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 318 KB
- Volume
- 394
- Category
- Article
- ISSN
- 0014-5793
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The gene, prs, encoding phosphoribosylpyrophosphate (PRPP) synthetase of Escherichia coli was isolated from a library of E. coli genes cloned in the bacteriophage 2D69 vector. A strain with a temperature-lethal defect in PRPP synthetase, (prs-2), was used as the host and cloning was performed by lys