𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Purification and characterization of the Escherichia coli thermoresistant gluconokinase encoded by the gntK gene

✍ Scribed by Hanae Izu; Osao Adachi; Mamoru Yamada


Book ID
115932850
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
318 KB
Volume
394
Category
Article
ISSN
0014-5793

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Isolation and characterization of the th
✍ Eugenio I. Vivas; Andreina Liendo; Karl Dawidowicz; TomΓ‘s IstΓΊriz πŸ“‚ Article πŸ“… 1994 πŸ› John Wiley and Sons 🌐 English βš– 423 KB

It is known that two gluconokinases are inducibly expressed during the utilization of gluconate by E. coli. One is therrnoresistant (activity stable for 3 h at 30 "C) and the other thermosensitive (losses 75% or more of its activity under the above conditions). The thermoresistant gluconokinase (EC

Mutations affecting gluconate catabolism
✍ Usua D. De Rekarte; Marieen CortΓ©s; Antonietta Porco; Gustavo NiΓ±o; Prof. TomΓ‘s πŸ“‚ Article πŸ“… 1994 πŸ› John Wiley and Sons 🌐 English βš– 500 KB

Media: Mineral medium (MM; TANAKA et ul. 1967) plus 5 pg ml-' of thiamine hydrochloride, 20 pg ml-' of L-amino acids as required (except for homoserine; 80 pg mi-'), and the carbon source at 2 gl-', was used. This medium was supplemented, when necessary, with 15 pg of tetracycline-HC1 and/or 500 pg

Cloning and characterization of the prs
✍ Hove-Jensen, Biarne πŸ“‚ Article πŸ“… 1985 πŸ› Springer 🌐 English βš– 865 KB

The gene, prs, encoding phosphoribosylpyrophosphate (PRPP) synthetase of Escherichia coli was isolated from a library of E. coli genes cloned in the bacteriophage 2D69 vector. A strain with a temperature-lethal defect in PRPP synthetase, (prs-2), was used as the host and cloning was performed by lys