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Mutations affecting gluconate catabolism in Escherichia coli. Genetic mapping of loci for the low affinity transport and the thermoresistant gluconokinase

✍ Scribed by Usua D. De Rekarte; Marieen Cortés; Antonietta Porco; Gustavo Niño; Prof. Tomás Istúriz


Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
500 KB
Volume
34
Category
Article
ISSN
0233-111X

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✦ Synopsis


Media: Mineral medium (MM; TANAKA et ul. 1967) plus 5 pg ml-' of thiamine hydrochloride, 20 pg ml-' of L-amino acids as required (except for homoserine; 80 pg mi-'), and the carbon source at 2 gl-', was used. This medium was supplemented, when necessary, with 15 pg of tetracycline-HC1 and/or 500 pg of DL-r-E-diaminopimelic acid (DAPA); also. when requircd, LURIA broth (Lb) plates,