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Purification and Characterization of S1 Mutants of α-Lytic Protease Having Altered Catalytic Properties

✍ Scribed by K.D. Haggett; L.D. Graham; S.J. Milner; R.G. Whittaker


Book ID
115565170
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
955 KB
Volume
314
Category
Article
ISSN
0003-9861

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A single-step procedure is small scale (annrox. 5mg) described for purifying a-lytic protease and its mutants on a from cell-free supematants of recombinant E. coli. Although free of non-enzyme pro&t, these preparations often contain fragments that arise from enzymatic self-digestion. Two strong cat