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Purification and Characterization of Recombinant Tomato Fruit (Lycopersicon esculentumMill.) Fructokinase Expressed inEscherichia coli

✍ Scribed by Eleazar Martinez-Barajas; Bradley M. Krohn; David M. Stark; Douglas D. Randall


Book ID
115645198
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
666 KB
Volume
11
Category
Article
ISSN
1046-5928

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The recently cloned cDNA for pea chloroplast thioredoxin f was used to produce, by PCR, a fragment coding for a protein lacking the transit peptide. This cDNA fragment was subcloned into a pET expression vector and used to transform E. coli cells. After induction with IPTG the transformed cells prod