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Purification and characterization of novel raw-starch-digesting and cold-adapted α-amylases from Eisenia foetida

✍ Scribed by Mitsuhiro Ueda; Tomohiko Asano; Masami Nakazawa; Kazutaka Miyatake; Kuniyo Inouye


Book ID
116345422
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
345 KB
Volume
150
Category
Article
ISSN
1096-4959

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The present study was aimed to express in Escherichia coli of an extracellular lipase from a local soil isolate. The lipase gene from soil isolate bacillus subtilis strain was cloned previously. The cloned gene in pTZ57R was digested out using NdeI and BamHI restriction enzymes. The extracted insert