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Molecular cloning, overexpression and characterization of the raw-starch-digestingα-amylase ofBacillus amyloliquefaciens

✍ Scribed by Dhanya Gangadharan; Priya Ramachandran; Gunasekaran Paramasamy; Ashok Pandey; K. Madhavan Nampoothiri

Book ID
111492372
Publisher
Versita
Year
2010
Tongue
English
Weight
469 KB
Volume
65
Category
Article
ISSN
0006-3088

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The present study was aimed to express in Escherichia coli of an extracellular lipase from a local soil isolate. The lipase gene from soil isolate bacillus subtilis strain was cloned previously. The cloned gene in pTZ57R was digested out using NdeI and BamHI restriction enzymes. The extracted insert