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Purification and characterization of intestinal adenosine deaminase from mice

โœ Scribed by Lisam Singh; Ramesh Sharma

Book ID
110247612
Publisher
Springer
Year
2000
Tongue
English
Weight
122 KB
Volume
204
Category
Article
ISSN
0300-8177

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โœ J. J. Centelles; Dr. R. Franco; J. Bozal ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 812 KB

Rat brain adenosine deaminase (E.C. 3.5.4.4.) was purified 667-fold from the supernatant fraction by the following techniques: heat treatment (60ยฐC), fractionation with ammonium sulfate, column chromatography on DEAE-Sepharose, and preparative gel electrophoresis. The purified enzyme was homogeneous