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Purification and Characterization of Glucose-6-Phosphate-Dehydrogenase from Cryptococcus neoformans: Identification as "Nothing Dehydrogenase"

✍ Scribed by W.G. Niehaus; T.C. Mallett


Book ID
115565142
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
481 KB
Volume
313
Category
Article
ISSN
0003-9861

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Glucose-6-phosphate dehydrogenase was purified to homogeneity from testes and kidneys of the inbred strain of mice (DBA/2J) by a simple two-step affinity column procedure. This involved the sequential application of 8-(6-aminohexyl)-amino-AMP- and -2', 5'-ADP-Sepharose columns and biospecific elutio