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Purification and characterization of epidermal growth factor (β-urogastrone) and epidermal growth factor fragments from large volumes of human urine

✍ Scribed by Charles D. Mount; Thomas J. Lukas; David N. Orth

Book ID
115706367
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
895 KB
Volume
240
Category
Article
ISSN
0003-9861

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Human recombinant EGF, secreted into the extracellular medium by E. c&i cells, was purified by a combination of solid phase extraction and HPLC. Using these techniques, the peptide was purified 122-fold, with a recovery of greater than 75%. The purified hEGF manifested no contaminating protein bands