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Purification and characterization of biotin synthase: An enzyme containing an iron—sulfur center.

✍ Scribed by Indrajit Sanval; Dennis Flint

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
83 KB
Volume
51
Category
Article
ISSN
0162-0134

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An avidin monomer affinity column for th
An avidin monomer affinity column for the purification of biotin-containing enzymes
✍ Katherine P. Henrikson; S.H.George Allen; W.Lee Maloy 📂 Article 📅 1979 🏛 Elsevier Science 🌐 English ⚖ 316 KB

A procedure is given for the preparation of an avidin monomer affinity column which is useful in the purification of biotin-containing enzymes. Both the propionyl-CoA carboxylase of Mycobacterium smegmatis and the methylmalonyl-CoA pyruvate transcarboxylase (EC 2.1.3.1) ofArachniapropionica and Prop