Purification and characterization of an extremely thermostable β-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus
✍ Scribed by Servé W. M. KENGEN; Evert J. LUESINK; Alfons J. M. STAMS; Alexander J. B. ZEHNDER
- Book ID
- 115129943
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 878 KB
- Volume
- 213
- Category
- Article
- ISSN
- 1432-1327
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A genomic library of the hyperthermophilic archaeon Pyrococcus furiosus was constructed in Escherichia coli using pBluescript II SK(+) as a cloning vector. One positive clone exhibiting thermophilic ester-hydrolyzing activity was directly detected by an in situ plate assay using the chromogenic subs
## Abstract The chaperonin molecular machine from hyperthermophilic archaeon __Pyrococcus furiosus__ was studied in this paper. The __Pyrococcus furiosus__ chaperonin gene (PfCPN) was amplified by PCR from the __Pyrococcus furiosus__ genomic DNA, and expressed in __Escherichia coli__ BL21‐Codonplus