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An extremely thermostable aromatic aminotransferase from the hyperthermophilic archaeon Pyrococcus furiosus

✍ Scribed by Giuseppina Andreotti; Maria Vittoria Cubellis; Gianpaolo Nitti; Giovanni Sannia; Xuhong Mai; Michael W.W. Adams; Gennaro Marino


Book ID
113269289
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
626 KB
Volume
1247
Category
Article
ISSN
0167-4838

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A genomic library of the hyperthermophilic archaeon Pyrococcus furiosus was constructed in Escherichia coli using pBluescript II SK(+) as a cloning vector. One positive clone exhibiting thermophilic ester-hydrolyzing activity was directly detected by an in situ plate assay using the chromogenic subs