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Purification and characterization of an enantioselective arylacetonitrilase fromPseudomonas putida

โœ Scribed by Anirban Banerjee; Praveen Kaul; U. C. Banerjee

Publisher
Springer
Year
2005
Tongue
English
Weight
378 KB
Volume
184
Category
Article
ISSN
0302-8933

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The e-glucosidase (C~-D-glucoside glucohydrolase, EC 3.2.1.20) of Pseudomonas fluorescens W was partially purified by (NH4)2SO4 fractionation, Sepha-dexG-200 and DEAE-cellulose column chromatography. The enzyme showed great specificity for maltose hydrolysis, with very little action against polymeri