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Purification and Characterization of a Phytase fromPseudomonas syringaeMOK1

โœ Scribed by Jaie Soon Cho; Chang Whan Lee; Seung Ha Kang; Jae Cheon Lee; Jin Duck Bok; Yang Soo Moon; Hong Gu Lee; Sung Chan Kim; Yun Jaie Choi


Publisher
Springer
Year
2003
Tongue
English
Weight
155 KB
Volume
47
Category
Article
ISSN
0343-8651

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Partial purification and characterizatio
โœ Arthur A. Guffanti; W. A. Corpe ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Springer ๐ŸŒ English โš– 688 KB

The e-glucosidase (C~-D-glucoside glucohydrolase, EC 3.2.1.20) of Pseudomonas fluorescens W was partially purified by (NH4)2SO4 fractionation, Sepha-dexG-200 and DEAE-cellulose column chromatography. The enzyme showed great specificity for maltose hydrolysis, with very little action against polymeri