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Purification and characterization of a β-glucosidase from solid-state cultures of Humicola grisea var. thermoidea

✍ Scribed by Filho, Edivaldo Ximenes Ferreira

Book ID
120020140
Publisher
NRC Research Press
Year
1996
Tongue
French
Weight
656 KB
Volume
42
Category
Article
ISSN
0008-4166

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An extracellular alkaline phosphatase (EC 3.1.3.1) was purified from conidia of Humicola grisea var. thermoidea. The molecular mass of the purified native enzyme was estimated as 126 kDa by gel filtration. SDS-PAGE revealed a single polypeptide band of 63 kDa, suggesting that the native enzyme was a