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Purification and biochemical characterization of β-xylosidase from Humicola grisea var. thermoidea

✍ Scribed by Elzira Maria de Almeida; Maria de Lourdes T.M. Polizeli; Héctor Francisco Terenzi; João Atilio Jorge

Book ID
109320675
Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
406 KB
Volume
130
Category
Article
ISSN
0378-1097

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Characterization of a conidial alkaline
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✍ Luciana Baracchini Buainain; Marina Kimiko Kadowaki; Maria de Lourdes Polizeli; 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 152 KB

An extracellular alkaline phosphatase (EC 3.1.3.1) was purified from conidia of Humicola grisea var. thermoidea. The molecular mass of the purified native enzyme was estimated as 126 kDa by gel filtration. SDS-PAGE revealed a single polypeptide band of 63 kDa, suggesting that the native enzyme was a