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Purification and characterization of 5′-methylthioadenosine phosphorylase from the hyperthermophilic archaeonPyrococcus furiosus

✍ Scribed by Giovanna Cacciapuoti; Costanzo Bertoldo; Assunta Brio; Vincenzo Zappia; Marina Porcelli

Publisher
Springer
Year
2003
Tongue
English
Weight
391 KB
Volume
7
Category
Article
ISSN
1431-0651

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## Abstract The chaperonin molecular machine from hyperthermophilic archaeon __Pyrococcus furiosus__ was studied in this paper. The __Pyrococcus furiosus__ chaperonin gene (PfCPN) was amplified by PCR from the __Pyrococcus furiosus__ genomic DNA, and expressed in __Escherichia coli__ BL21‐Codonplus