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Characterization of ATPase activity of class II chaperonin from the hyperthermophilic archaeonPyrococcus furiosus

✍ Scribed by Hua-you Chen; Xiao-li Tan; Jian Lu; Chun-xia Zhang; Yi Zhang; Sheng-li Yang

Publisher
Springer Netherlands
Year
2009
Tongue
English
Weight
342 KB
Volume
31
Category
Article
ISSN
0141-5492

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## Abstract The chaperonin molecular machine from hyperthermophilic archaeon __Pyrococcus furiosus__ was studied in this paper. The __Pyrococcus furiosus__ chaperonin gene (PfCPN) was amplified by PCR from the __Pyrococcus furiosus__ genomic DNA, and expressed in __Escherichia coli__ BL21‐Codonplus