Purification and characterisation of a mollusc β-d-(1→3)-glucan hydrolase
✍ Scribed by M.G. Lindley; R.S. Shallenberger; S.M. Herbert
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 480 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0308-8146
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A P-D-glucan isolated from a Japanese marine alga known as "Arame" (Eisenia bicyclis) was shown by methylation analysis to be composed of (l-3) and (l-+6) linkages and ( 1+3),(1+6) branch points. The 13C-n.m.r. spectrum confirmed these linkages and also verified the absence of /3-( l-+4) linkages. T
A highly sensitive enzyme-linked immunosorbent assay specific to (1→3)-β-Dglucans (GBP-ELISA) has been developed using a novel (1→3)-β-D-glucan-binding protein (T-GBP), which was purified from the amebocyte lysate of the Japanese horseshoe crab, Tachypleus tridentatus. This method allowed quantitati