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Purification and biochemical characterisation of a novel glutamate decarboxylase from rice bran

✍ Scribed by Li Wang; Dong Xia Xu; Ying Guo Lv; Hui Zhang


Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
169 KB
Volume
90
Category
Article
ISSN
0022-5142

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A novel type of monoamine oxidase (EC. 1.4.3.4) present in the liver of skipjack tuna (Katsuwonus pelamis) was extracted from mitochondrial preparations by Triton X-100. The enzyme was puriÐed by ammonium sulphate fractionation, followed by Sephadex G-200, butyl-toyopearl 650 M, phenyl-toyopearl 650