A Novel Type Mitochondrial Monoamine Oxidase from the Liver of Skipjack Tuna (Katsuwonus pelamis): Purification and Characterisation
✍ Scribed by Nagai, Takeshi; Hamada, Moritsugu; Kai, Norihisa; Tanoue, Yasuhiro; Nagayama, Fumio
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 237 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
A novel type of monoamine oxidase (EC. 1.4.3.4) present in the liver of skipjack tuna (Katsuwonus pelamis) was extracted from mitochondrial preparations by Triton X-100. The enzyme was puriÐed by ammonium sulphate fractionation, followed by Sephadex G-200, butyl-toyopearl 650 M, phenyl-toyopearl 650 M and hydroxyapatite chromatography. The molecular weight of the enzyme was estimated to be about 110 000 by gel Ðltration on Sephadex G-200. The enzyme was activated by Mn2, but was inhibited by Cu2, Zn2and pchloromercuribenzoic acid (PCMB). Furthermore, the enzyme was completely inhibited by Hg2and 5,5@-dithiobis(2-nitrobenzoic acid) (DTNB).