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Purification and activity standardisation of a 166mHo solution

✍ Scribed by Youcef Nedjadi; Philippe Spring; Claude Bailat; P. Froidevaux; C. Wastiel; François Bochud


Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
255 KB
Volume
66
Category
Article
ISSN
0969-8043

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Prostaglandin H synthetase (PGH synthetase) has been purified to homogeneity from sheep vesicular glands. The pure enzyme has a specific activity of about 40 @.I of arachidonic acid consumed per minute per milligram of protein, which corresponds to a turnover number of 2800 mitt-' per subunit. The p