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Pulsed field gradient nmr determination of the temperature dependence of the tracer diffusion coefficient of hemoglobin

✍ Scribed by Cherie H. Everhart; Charles S. Johnson Jr.

Publisher
Wiley (John Wiley & Sons)
Year
1982
Tongue
English
Weight
339 KB
Volume
21
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

We have studied the temperature dependence of the tracer diffusion coefficient of carbonmonoxy hemoglobin A (HbA‐CO) by means of pulsed‐field gradient nmr (PFG‐nmr). Measurements were made over the temperature range from 15 to 35Β°C for samples having concentrations 7.4 and 16.7 g/dL. No significant deviations were found from the predictions of the Stokes‐Einstein relation. Thus, this work does not corroborate the recently proposed conformational change in hemoglobin at 22Β°C. The advantages of PFG‐nmr for the study of hemoglobin are discussed.

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