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Pulse schemes for the measurement of3JC′Cγ and3JNCγ scalar couplings in15N,13C uniformly labeled proteins

✍ Scribed by Robert Konrat; D.R. Muhandiram; N.A. Farrow; Lewis E. Kay


Book ID
110369389
Publisher
Springer Netherlands
Year
1997
Tongue
English
Weight
307 KB
Volume
9
Category
Article
ISSN
0925-2738

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A JCH-Modulated 2D (HACACO)NH Pulse Sche
✍ T.Kevin Hitchens; Scott A. McCallum; Gordon S. Rule 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 73 KB

A triple-resonance pulse sequence is presented for the quantitative measurement of 1 H ␣ -13 C ␣ single-bond couplings in 15 N, 13 C uniformly labeled proteins. This 1 J CH -modulated (HACACO)NH experiment yields 1 H N -15 N-correlated 2D spectra in which the amplitude of each peak is modulated by t