Transposon donor plasmids, based on ColI
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Boulnois, Graham J. ;Varley, Jennifer M. ;Sharpe, Geoffrey S. ;Franklin, F. Chri
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Article
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1985
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Springer
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English
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The properties of pLG221, a derivative of the ColIb plasmid carrying the transposon Tn5 are described. This plasmid can be used to introduce Tn5 by conjugation from Escherichia coli into a variety of Gram negative bacteria outside the host range for maintenance of ColIb. Plasmid pLG221, and a simila