✦ LIBER ✦
Transposon donor plasmids, based on ColIb-P9, for use in Pseudomonas putida and a variety of other Gram negative bacteria
✍ Scribed by Boulnois, Graham J. ;Varley, Jennifer M. ;Sharpe, Geoffrey S. ;Franklin, F. Christopher H.
- Book ID
- 104748652
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 598 KB
- Volume
- 200
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
✦ Synopsis
The properties of pLG221, a derivative of the ColIb plasmid carrying the transposon Tn5 are described. This plasmid can be used to introduce Tn5 by conjugation from Escherichia coli into a variety of Gram negative bacteria outside the host range for maintenance of ColIb. Plasmid pLG221, and a similar plasmid pLG223 carrying TnlO may be of general utility as vectors for transposon-mediated mutagenesis in a variety of Gram negative bacteria.