The possibility that small nuclear RNA species U1 might be involved in the inhibition of protein synthesis that occurs during mitosis has been explored. Upon exposure of mitotic HeLa cell extracts to 1% sodium deoxycholate, the majority of the rapidly sedimenting U1 RNA shifted to lower sedimentatio
Pseudourine formation in small nuclear RNAs
โ Scribed by J.R. Patton
- Book ID
- 116121790
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 374 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0300-9084
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Several observations have been made about the associations of small nuclear RNAs (snRNAs) in human cells. When nuclear RNA was extracted with phenol and chloroform under standard nondenaturing conditions, the proportion of the nuclear snRNA content that cosedimented with high molecular weight RNA wa
We have examined some aspects of the biosynthesis of human small nuclear RNAs (snRNAs). The sensitivity of U5 and U4 snRNA synthesis to a-amanitin in whole cells suggests that RNA polymerase I I is involved in the synthesis of these RNA species, in addition to that of U1, U2, and U3 snRNA. Two RNA b