𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Associations of small nuclear RNAs in human cells

✍ Scribed by James H. Smith; George L. Eliceiri

Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
938 KB
Volume
117
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Several observations have been made about the associations of small nuclear RNAs (snRNAs) in human cells. When nuclear RNA was extracted with phenol and chloroform under standard nondenaturing conditions, the proportion of the nuclear snRNA content that cosedimented with high molecular weight RNA was very low. These results do not support the proposal that it is a large percentage of the cellular snRNA content that is involved in relatively stable base-paired interactions with heterogeneous nuclear RNA at any given time. The various small nuclear ribonucleoprotein particles (snRNPs), in which the snRNAs are found in t h e cell, appear to differ substantially in their sedimentation rates under conditions of physiological ionic strength. Using anti-RNP and anti-Sm antibodies to analyze various subcellular fractions, we found that most, if not all, of the U1 snRNA cellular content is associated with the polypeptide(s) bearing the RNP determinant (in interphase and mitotic cells) and with the polypeptide(s) carrying the Sm determinant (in mitotic cells).

πŸ“œ SIMILAR VOLUMES

Biosynthesis of small nuclear RNAs in hu
Biosynthesis of small nuclear RNAs in human cells
✍ Settara C. Chandrasekharappa; James H. Smith; George L. Eliceiri πŸ“‚ Article πŸ“… 1983 πŸ› John Wiley and Sons 🌐 English βš– 773 KB

We have examined some aspects of the biosynthesis of human small nuclear RNAs (snRNAs). The sensitivity of U5 and U4 snRNA synthesis to a-amanitin in whole cells suggests that RNA polymerase I I is involved in the synthesis of these RNA species, in addition to that of U1, U2, and U3 snRNA. Two RNA b

Metabolism of small RNAs in cultured hum
Metabolism of small RNAs in cultured human cells
✍ Kanakendu Choudhury; Indrani Choudhury; George L. Eliceiri πŸ“‚ Article πŸ“… 1989 πŸ› John Wiley and Sons 🌐 English βš– 611 KB

There are gaps in what is known about the metabolism of some mammalian small RNA species. Our present observations can be summarized as follows. The level of radiolabeled mature U1 KNA doubled between 2 and 24 hr of label chase, while that of all other small RNA species tested did not change. These

Small nuclear rnas in cellular growth an
Small nuclear rnas in cellular growth and differentiation. I: Metabolic alterations seen in friend erythroleukemic cells
✍ Gerald S. Kuncio; Lester Goldstein πŸ“‚ Article πŸ“… 1981 πŸ› John Wiley and Sons 🌐 English βš– 663 KB

## Abstract Electrophoretic analysis of near steady‐state labeled nuclear RNA obtained from Friend virus‐transformed murine erythroleukemic cells reveals the presence of at least 15 small nuclear RNAs (snRNAs) distinct from ribosomal 5.8S or 5S. Identical __qualitative__ distributions were obtained

Synthesis of the small molecular weight
Synthesis of the small molecular weight monodisperse nuclear RNA's in contact-inhibited cell cultures
✍ Alan Rein; Sheldon Penman πŸ“‚ Article πŸ“… 1972 πŸ› John Wiley and Sons 🌐 English βš– 287 KB

The small molecular weight monodisperse nuclear RNA's are synthesized in contact-inhibited cultures of 3T3 cells. The level of synthesis of these RNA's, and of ribosomal and transfer RNA's, appears to be oiily 8-20% of that observed in growing cultures. The synthesis of all of these relatively stabl

Ultraviolet light-induced inhibition of
Ultraviolet light-induced inhibition of small nuclear RNA synthesis
✍ Brian P. Eliceiri; Kanakendu Choudhury; Quincy O. Scott; George L. Eliceiri πŸ“‚ Article πŸ“… 1989 πŸ› John Wiley and Sons 🌐 English βš– 656 KB

## Lorrrs Mizzouri 63 104 Two apparently distinct types of inhibition of the synthesis of U1, U2, U3, U4, and US small nuclear KNA, induced by ultraviolet (UV) radiation, have been described before: immediate and delaycd. O u r present observations can be summarized as follows: a) neither the imme