In order to study the accumulation and transport of rosmarinic acid in suspension cells of Coleus blumei we established an efficient method to isolate protoplasts and vacuoles. Protoplasts were disrupted by an osmotic shock in a medium with basic pH containing ethylenediamine tetraacetic acid. The r
β¦ LIBER β¦
Protoplast Isolation from Euphorbia tirucalli L. Cell Suspension Cultures and Sustained Cell Division
β Scribed by Ohyama, Kanji; Misawa, Norihiko; Yamano, Yoshiaki; Komano, Tohru
- Book ID
- 123010124
- Publisher
- Elsevier
- Year
- 1984
- Weight
- 985 KB
- Volume
- 113
- Category
- Article
- ISSN
- 0044-328X
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