๐”– Bobbio Scriptorium
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Protoplast isolation and callus production from leaves of tissue-culturedVitisspp.

โœ Scribed by Ni Lee; Hazel Y. Wetzstein

Publisher
Springer
Year
1988
Tongue
English
Weight
724 KB
Volume
7
Category
Article
ISSN
0721-7714

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Yields of 106-108 peach mesophyll cells and protoplasts-gfw -1 were obtained depending on factors such as digesting enzymes, and leaf size. Onozuka R-10 (2%) in combination with Macerase (0.5%) was found best for protoplast isolation and mediocre for cell isolation among several enzyme combinations