Protoplast fusion of Schizosaccharomyces pombe auxotrophic mutants of identical mating-type

✍ Scribed by Sipiczki, M. ;Ferenczy, L.

• Publisher: Springer
• Year: 1977
• Tongue: English
• Weight: 455 KB
• Volume: 151
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00446915

✦ Synopsis

Protoplasts of methionine- and lysine-requiring h- mutants isolated from the L972 h- strain of Schizosaccharomyces pombe were fused. The protoplasts were obtained from the cells with enzymes produced by Trichoderma viride. When a mixture of the protoplasts was treated with 30% PEG 4000 solution containing 10 mM CaCl2, cell fusion and complementation was attained with a frequency of 0.17%. Both fusion partners were recovered among the spores after crossing of the fusion products with the strain M210 ade6 h+. Cytological and haploidization examinations showed that the fusion cells are not heterokaryons, and that the increased amount of genetic material is situated in one nucleus.