✦ LIBER ✦

Strains of Schizosaccharomyces pombe with a disrupted swi1 gene still show some mating-type switching

✍ Scribed by Schmidt, Henning

Publisher: Springer
Year: 1987
Tongue: English
Weight: 533 KB
Volume: 210
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00327201

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✦ Synopsis

The swi1+ gene is necessary for effective mating-type (MT) switching in Schizosaccharomyces pombe. It was cloned on a 4.2 kb genomic DNA fragment. By site-directed integration into the genome and gene disruption experiments it was proved that the swi1+ gene itself and not a suppressor had been isolated. Disruption of the swi1+ gene causes a phenotype identical to that of the original swi1 mutant, i.e. the strain still shows some MT switching. The swi1 gene is unique in the genome and gives rise to a 3 kb mRNA.