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Protoplast fusion in apetite-negative yeast,Kluyveromyces lactis

✍ Scribed by Andrew J. Morgan; Aurora Brunner; Peter A. Whittaker

Publisher: Springer-Verlag
Year: 1980
Tongue: English
Weight: 634 KB
Volume: 2
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00445699

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✦ Synopsis

The technique of protoplast fusion has been applied to the problem of unstable diploidy in the yeast Kluyveromyces lactis. By protoplast fusion between heterothallic strains of like mating-type, sporulationdeficient hybrids can be obtained. Biochemical, cytological, and genetical characterisation of these hybrids suggests that the majority of fusion products are diploid. Sporulating hybrids can be constructed by protoplast fusion between homothallic strains. Tetrad analysis of these hybrids demonstrates conclusively the diploid nature of fusion products.

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