Proton Detection of Choline and Lactate in EMT6 Tumors by Spin-Echo-Enhanced Selective Multiple-Quantum-Coherence Transfer

✍ Scribed by Qiuhong He; Zaver M. Bhujwalla; Jerry D. Glickson

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 416 KB
• Volume: 112
• Category: Article
• ISSN: 1064-1866
• DOI: 10.1006/jmrb.1996.0104

✦ Synopsis

An extension of the

Sel-MQC pulse sequence-SEE-SelMQC dominate the spectrum over the entire proton chemical-shift (spin-echo-enhanced selective multiple-quantum coherence transrange. Complete suppression of lipid and water in fatty tisfer) -that completely suppresses lipid and water in tissues consues, especially in the human breast, where the lipid signals taining mobile lipid in a single scan and detects 1 H resonances of are more than ten times as intense as the water signal (14), multiple metabolites is described. As in the Sel-MQC lactate-editis difficult yet essential for detection of pathologies and for ing experiments [Q. He et al., J. Magn. Reson. B 106, 203 (1995)], monitoring therapeutic response of human cancer. SEE-SelMQC acquires lactate from its ZQ r DQ coherence-trans-''J-coupling filters''-difference spectroscopy (15-19), fer pathway; in addition, the method recovers signal from other polarization transfer (20-23), and multiple-quantum (MQ) metabolites by selective generation of additional spin echoes using coherence-transfer (24-34) techniques-designed to selecextra frequency-selective pulses and gradients. This method introtively detect lactate (or any metabolite with coupled spins) duces no loss of lactate signal intensity beyond the 50% that is have partially eliminated lipid and water. However, some of lost through the multiple-quantum coherence-transfer process.

The spatial distributions of choline and lactate with a phantom the coupled spins of the lipid molecules may generate MQ and in vivo, in subcutaneously implanted murine EMT6 tumors, coherences that pass through the J-coupling filter and conhave been simultaneously mapped.