𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Proton and phosphorus nmr investigation of the conformational states of acid polyadenylic double helix

✍ Scribed by Deborah B. Lerner; David R. Kearns

Publisher
Wiley (John Wiley & Sons)
Year
1981
Tongue
English
Weight
689 KB
Volume
20
Category
Article
ISSN
0006-3525

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Proton and phosphorus nmr have been used to investigate the double‐helical structures of polyriboadenylic acid [poly(A)] formed in acidic solutions (pH < 6). The results obtained at low pH (∼4.5) are consistent with the model for the acid poly(A) double helix proposed by Rich [Rich, A., Davies, D. R., Crick, F. H. C. & Watson, J. D. (1961) J. Mol. Biol. 3, 71–86]. Other models that have been proposed are inconsistent with the nmr data. The nmr measurements have also been used to examine the conformation of poly(A) helix in the half‐protonated state. Although the base‐stacking arrangement of this state is similar to that observed in the more extensively protonated low‐pH state, the phosphate backbone conformation is different from that found in either the neutral or low‐pH structures.

📜 SIMILAR VOLUMES

Conformation of the Glycosidic Linkage i
Conformation of the Glycosidic Linkage in a Disaccharide Investigated by Double-Quantum Solid-State NMR
✍ S. Ravindranathan; T. Karlsson; K. Lycknert; G. Widmalm; M.H. Levitt 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 116 KB

Double-quantum heteronuclear local field NMR is performed on a sample of a 13C2-labeled disaccharide, in which the two 13C spins are located on opposite sides of the glycosidic linkage. The evolution of the double-quantum coherences is found to be consistent with the solid-state conformation of the

Examination of formamide, formamidic aci
Examination of formamide, formamidic acid, amidine dimers, and the double proton transfer transition states involving these dimers
✍ Theresa Julia Zielinski; Raymond Alcide Poirier 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 389 KB

Structures and relative energies were obtained for the hydrogen bonded dimers of formamide and formamidic acid using the 3-21G basis set. A double proton transfer transition state is claimed to link these two dimers. While the structure of the transition state was intermediate between those of the t

A Study of stepwise conformational chang
A Study of stepwise conformational changes in poly(β-benzyl-L-aspartate) with the helix–coil transition by means of proton and carbon-13 NMR
✍ Shigeru Sase; Yasuyuki Suzuki; Yoshio Inoue; Riichirô Chûjô 📂 Article 📅 1977 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 648 KB

The helix-coil transition for poly(P-benzyl-L-aspartate) [poly(Asp[OBzl])] in solvent mixtures of trifluoroacetic acid/deuterated chloroform (F3AcOH/CDC13) was studied by means of proton and carbon-13 nmr. Conformational fixation of the side chain occurs before the coil-helix transition of the backb