𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Protoheme extraction from plant tissue

✍ Scribed by Linda C. Stillman; Merrill L. Gassman

Book ID
102627056
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
452 KB
Volume
91
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A method for reproducibly estimating the protoheme content of plant tissues has been developed. The tissue sample is homogenized in 80% acetone to remove pigments and lipids; protoheme is then extracted from the tissue residue with 2% HCl in acetone and quantitatively transferred into diethyl ether. After evaporation of the ether, the residue is dissolved in alkaline pyridine, and the protoheme concentration is estimated from a dithionite-reduced-minus-ferricyanide-oxidized spectrum. When compared to some other methods, this procedure gives consistently higher yields.

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## Abstract Plant tissues usually contain high levels of proteases and secondary metabolites that severely interfere with protein extraction, separation, and identification. Preparation of high‐quality protein samples from plant tissues for proteomic analysis represents a great challenge. This arti