Proteomics meets cell biology: The establishment of subcellular proteomes

✍ Scribed by Eva Jung; Manfred Heller; Jean-Charles Sanchez; Denis F. Hochstrasser

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 88 KB
• Volume: 21
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/1522-2683(20001001)21:16<3369::aid-elps3369>3.0.co;2-7

✦ Synopsis

Proteome research aims to unravel the biological complexity encoded by the genome. Due to the complexity of higher eukaryotic cells, single-step characterization of a proteome is likely to be difficult to achieve. However, advantage can be taken of the macromolecular architecture of a cell, e.g., subcellular compartments, organelles, macromolecular structures and multiprotein complexes, to establish subcellular proteomes. This review highlights recent developments in this area of proteomics, namely the establishment of two-dimensional electrophoresis (2-DE) reference maps of subcellular compartments and organelles as well as the characterization of macromolecular structures and multiprotein complexes using a proteomics approach.