## Abstract It was found either in Western‐blot analysis or in indirect immunofluorescence microscopy that cells of the alga Chlamydomonas reinhardtii contain polypeptides cross‐reacting with antibodies directed against red blood cell spectrin. The protein could also be detected by immunoprecipitat
Proteins reacting with anti-spectrin antibodies are present in Chlamydomonas Cells.
✍ Scribed by Michal Lorenz; Brygida Bisikirska; Beata Hanus-Lorenz; Kazimierz Strzalka; Aleksander F. Sikorski
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 439 KB
- Volume
- 19
- Category
- Article
- ISSN
- 1065-6995
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✦ Synopsis
It was found either in Western‐blot analysis or in indirect immunofluorescence microscopy that cells of the alga Chlamydomonas reinhardhi contain polypeptides cross‐reactng with antibodies directed against red blood cell spectrin. The protein could also be detected by immunoprecipitation with anti‐spectrin antibodies. C. reinhardtii cells contain distinct polypeptide chains reacting with antibodies directed against either α‐ or β‐ spectrin subunits. This protein was extracted from the cells with low ionic strength solution but was not with nonionic detergent.
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## RESULTS. The relative uptake of G250 in NU-12 tumors was very high at low protein doses (125% injected dose/g [%ID/g]), but decreased at higher doses, suggesting